DNAgard Tissues & Cells

Collect, Ship and Store Tissues & Cells at Room Temperature.

DNAgard® is designed for immediate stabilization of DNA from intact cells and tissues with the convenience of room temperature shipping, processing and storage. The liquid storage reagent rapidly permeates cell membranes to stabilize and protect genomic DNA.

Technology Overview:


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Features & Benefits


Shipping at room temperature

  • No dry ice required
  • Reduced shipping cost
  • Avoid temperature fluctuations

Ideal for field collection and tissue harvesting

Optional dry-down feature allowing longer term storage

Compatible with all downstream applications

 

Stabiization of DNA at Room Temperature in a liquid format.

The aqueous storage reagent permeates cell membranes to stabilize and protect DNA for at least 6 months at room temperature (figure1). DNAgard protects DNA in a variety of tissues and cells (see figures 2 & 3).


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Figure 1. DNAgard protects DNA for at least 6 months in rat kidney tissues at room temperature.


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Figure 2. DNAgard protects DNA in a variety of tissues at room temperature.


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Figure 3. DNAgard protects DNA in mammalian cells at room temperature. On the right, DNA yield comparison between samples stored for 2 months in DNAgard and at -80°C


Broad range of applications

DNA protected using DNAgard is suitable for use in many downstream applications, including long-range PCR and Real Time PCR

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Figure 4. Successful real-time PCR and long-range PCR after 2 months storage in DNAgard and at -80°C .


Unique dry-down feature for longer storage at room temperature

For longer storage, samples can be dried down and stored at room temperature for at least 1 year* (*accelerated aging). Dry samples can be easily recovered by simple rehydratation and are ready for DNA extraction. The quality of DNA is preserved by DNAgard, whereas degradation is noticeable if preserved on FTA cards or left unprotected at room temperature (see figure 5).

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Figure 5. DNAgard protects DNA from degradation in mammalian cells stored dried at 50 degrees for 2 months (equivalent to 1 year at room temperature based on accelerated aging).